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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in many tumors, which leads to chromosome instability, and so may possibly lead to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential part in advertising mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are actually explained. Two more splice variants were being noted but comprehensive nucleotide sequence information is just not however out there.
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Blocking peptides are peptides that bind specifically for the goal antibody and block antibody binding. These peptide commonly incorporates the epitope regarded by the antibody.
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This guideline outlines even further attainable optimization of mobile and immunoassay parameters to ensure the best possible benefits are...
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This guidebook outlines even more doable optimization of mobile and immunoassay parameters to be sure the absolute best final results are...
Antibodies certain to the blocking peptide no more bind for the epitope within the concentrate on protein. By check here comparing the staining within the blocked antibody compared to the antibody by itself, you can see which staining is specific.
Goal Information Cyclin E belongs to the very conserved cyclin family, whose users are characterised by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Various cyclins exhibit unique expression and degradation patterns which contribute towards the temporal coordination of each and every mitotic celebration. Cyclin E sorts a fancy with and features like a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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